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fastcast gels  (Bio-Rad)


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    Structured Review

    Bio-Rad fastcast gels
    Fastcast Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgx+stain+free+fastcast+acrylamide+kit/TGX+Stain-Free+FastCast+Acrylamide+Kit/pm42129557-843-27-29
    Average 96 stars, based on 653 article reviews
    fastcast gels - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Tolerance mechanisms to UV-B in olive pollen demonstrate cultivar-dependent biochemical responses.
    Article Snippet: .. The TGX Stain-Free FastCast Acrylamide Kit (BioRad) was used for gel preparation according to the manufacturer's instructions. ..

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Ethanol (Cat# E7023) and complete protease inhibitor cocktail (Cat# P8340) were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. TGX Stain-Free FastCast Acrylamide Kit (10%; Cat# 1610183), Clarity Western ECL substrates (Cat# 1705061), polyvinylidene difluoride (PVDF) membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad (Hercules, CA, USA). .. The CellTiter-Glo 2.0 Cell Viability Assay kit (Cat# G9241) was obtained from Promega (Madison, WI, USA).

    Article Title: MSC Exosomes and Rutin‐Chitosan–Pectin Nanoparticles Synergize to Ameliorate Adjuvant Arthritis via Th1/Th2 Modulation, MMP Suppression, Nrf2 Upregulation, and Antioxidant Boost
    Article Snippet: Protein extraction was performed using the ReadyPrep Protein Isolation Kit (Bio‐Rad Inc., Hercules, CA, USA), and protein concentrations were determined with the Bradford assay (Bio Basic Inc., Markham, Ontario, Canada). .. Equal amounts of protein (20 μg) were mixed with Laemmli sample buffer and denatured by boiling at 95°C for 5 min. Proteins were separated by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS‐PAGE) using the TGX Stain‐Free FastCast Acrylamide Kit (Bio‐Rad Laboratories, Inc., Hercules, CA, USA). .. The proteins were transferred to a PVDF membrane using the Bio‐Rad Trans‐Blot Turbo system.

    Article Title: Exploring the inflammatory origins of gouty arthritis: mechanistic studies based on local lesion proteomics and validation
    Article Snippet: Ammonium bicarbonate (Sigma-Aldrich, A6141-500G), TEAB (Sigma-Aldrich, T7408-100mL), Urea (Amresco, M123-1KG), Protein Quantification Dye (Huaxingbio, HXJ5137), Bovine Serum Albumin (Thermo Scientific, 23209), Dithiothreitol DTT (Amresco, M109-5G), Iodoacetamide IAM (Amresco, M216-30G), Trypsin (Promega, V5280/100ug), Ziptip (Millipore, ZTC18M096), Acetonitrile (J.T.Baker, 34851 MSDS), Ammonia solution (Wako Pure Chemical Industries Ltd, 013-23355), Formic acid (Sigma-Aldrich, T79708 ), Sample vials (Thermo, 11190533), Vial caps (Thermo, 11150635). .. Plasma Low Abundance Enrichment Magnetic Bead Kit QLBIO MagicOmics-DMB8X, Blood Exosome Enrichment Magnetic Bead Kit QLBIO MagicOmics-EMB8X, Micro/Universal Proteomics Kit QLBIO MagicOmics-MMB8X, Proteomics Robot QLBIO MagicOmics-AP-96, TGX Stain-Free FastCast Acrylamide Kit(Bio-Rad, #1610183), Universal Antibody Dilution Buffer (Yamei PS119L), anti-arachidonate 15-lipoxygenase(anti-ALOX15,BBI LIFE SCIENCES CORPORATION D161433), anti-glutathione peroxidase 4(anti-GPX4,Bioswamp PAB47937 ), ferritin heavy chain 1(anti-FTH1,BBI LIFE SCIENCES CORPORATION D290553), goat anti-rabbit IgG (BBI LIFE SCIENCES CORPORATION D110058), ECL Chemiluminescent Reagent (Millipore WBKLS0500). ..

    Article Title: γ-Tocotrienol inhibition of galectin-3 expression, distribution and oligomerization in highly metastatic breast cancer cells.
    Article Snippet: Goat anti-mouse antibody (#NEF822001EA) used for Western blot analysis was purchased from PerkinElmer Biosciences (Boston, MA, USA). .. TGX Stain-Free FastCast Acrylamide Kit 12% (#1610185) was purchased from Bio-Rad (Hercules, CA, USA) for use in Stain-Free Western Blot analysis. .. Goat anti-rabbit and anti-mouse secondary antibodies used for immunocytochemistry were obtained from Invitrogen (Carlsbad, CA, USA).

    Article Title: Spliceosomal mutation drives melanoma tumorigenesis via lineage-specific RAS activation
    Article Snippet: Cells were seeded 48 hours before lysis and treated with 2% SDS (Sangon Biotech, no. B548118). .. Protein extracts were prepared by mixing with 6× SDS-PAGE Sample Loading Buffer (Beyotime, no. P0015F) and heating at 100°C for 10 min. Gels were cast using the TGX Stain-Free FastCast Acrylamide Kit, 10% (Bio-Rad, no. 1610183) ( ). .. Proteins were transferred using 1× transfer buffer prepared with a 1:1:3 dilution of water, absolute ethanol, and Trans-Blot Turbo 5× Transfer Buffer (Bio-Rad, no. 10026938) onto 0.22-μm Immun-Blot PVDF Membranes (Bio-Rad, no. 1620177).

    Article Title: Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programs
    Article Snippet: Pierce Micro BCA Protein Assay Kit , ThermoFisher Scientific , Cat# 23235. .. 10% TGX Stain-free FastCast Acrylamide Kit , BioRad , Cat1610183. ..

    Article Title: Design, expression, purification, and application of novel recombinant miR-491 molecules to define the biogenesis and function of miR-491-3p versus -5p in posttranscriptional regulation of UDP-glucuronosyltransferase 1A1.
    Article Snippet: Direct-zol RNA MiniPrep Kit (Cat# R2072) was bought from Zymo Research, and primers were synthesized by Integrated DNA Technologies. .. TGX Stain-Free FastCast Acrylamide Kit, 10% (Cat# 1610183), Clarity Western ECL Substrates (Cat# 1705061), polyvinylidene difluoride membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad. .. Monoclonal anti-UGT1A1 antibody (Cat# A22609) was obtained from ABclonal Science, Inc. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat# 2118L) was purchased from Cell Signaling Technology, Inc. Anti-ß-actin antibody (Cat# A5441), ßestradiol 17-(β-D-glucuronide) sodium salt (Cat# E1127), 17- ethynylestradiol (Cat# E4876), and alamethicin (Cat# E1127) were obtained from MilliporeSigma. ß-Estradiol 3-(β-D-glucuronide) sodium salt (Cat# HY-W585842) was purchased from MedChemExpress LLC.

    Western Blot:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Ethanol (Cat# E7023) and complete protease inhibitor cocktail (Cat# P8340) were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. TGX Stain-Free FastCast Acrylamide Kit (10%; Cat# 1610183), Clarity Western ECL substrates (Cat# 1705061), polyvinylidene difluoride (PVDF) membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad (Hercules, CA, USA). .. The CellTiter-Glo 2.0 Cell Viability Assay kit (Cat# G9241) was obtained from Promega (Madison, WI, USA).

    Article Title: γ-Tocotrienol inhibition of galectin-3 expression, distribution and oligomerization in highly metastatic breast cancer cells.
    Article Snippet: Goat anti-mouse antibody (#NEF822001EA) used for Western blot analysis was purchased from PerkinElmer Biosciences (Boston, MA, USA). .. TGX Stain-Free FastCast Acrylamide Kit 12% (#1610185) was purchased from Bio-Rad (Hercules, CA, USA) for use in Stain-Free Western Blot analysis. .. Goat anti-rabbit and anti-mouse secondary antibodies used for immunocytochemistry were obtained from Invitrogen (Carlsbad, CA, USA).

    Article Title: Design, expression, purification, and application of novel recombinant miR-491 molecules to define the biogenesis and function of miR-491-3p versus -5p in posttranscriptional regulation of UDP-glucuronosyltransferase 1A1.
    Article Snippet: Direct-zol RNA MiniPrep Kit (Cat# R2072) was bought from Zymo Research, and primers were synthesized by Integrated DNA Technologies. .. TGX Stain-Free FastCast Acrylamide Kit, 10% (Cat# 1610183), Clarity Western ECL Substrates (Cat# 1705061), polyvinylidene difluoride membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad. .. Monoclonal anti-UGT1A1 antibody (Cat# A22609) was obtained from ABclonal Science, Inc. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat# 2118L) was purchased from Cell Signaling Technology, Inc. Anti-ß-actin antibody (Cat# A5441), ßestradiol 17-(β-D-glucuronide) sodium salt (Cat# E1127), 17- ethynylestradiol (Cat# E4876), and alamethicin (Cat# E1127) were obtained from MilliporeSigma. ß-Estradiol 3-(β-D-glucuronide) sodium salt (Cat# HY-W585842) was purchased from MedChemExpress LLC.

    SYBR Green Assay:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Ethanol (Cat# E7023) and complete protease inhibitor cocktail (Cat# P8340) were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. TGX Stain-Free FastCast Acrylamide Kit (10%; Cat# 1610183), Clarity Western ECL substrates (Cat# 1705061), polyvinylidene difluoride (PVDF) membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad (Hercules, CA, USA). .. The CellTiter-Glo 2.0 Cell Viability Assay kit (Cat# G9241) was obtained from Promega (Madison, WI, USA).

    Article Title: Design, expression, purification, and application of novel recombinant miR-491 molecules to define the biogenesis and function of miR-491-3p versus -5p in posttranscriptional regulation of UDP-glucuronosyltransferase 1A1.
    Article Snippet: Direct-zol RNA MiniPrep Kit (Cat# R2072) was bought from Zymo Research, and primers were synthesized by Integrated DNA Technologies. .. TGX Stain-Free FastCast Acrylamide Kit, 10% (Cat# 1610183), Clarity Western ECL Substrates (Cat# 1705061), polyvinylidene difluoride membranes (Cat# 1620184), and iTaq Universal SYBR Green Supermix (Cat# 1725124) were purchased from Bio-Rad. .. Monoclonal anti-UGT1A1 antibody (Cat# A22609) was obtained from ABclonal Science, Inc. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat# 2118L) was purchased from Cell Signaling Technology, Inc. Anti-ß-actin antibody (Cat# A5441), ßestradiol 17-(β-D-glucuronide) sodium salt (Cat# E1127), 17- ethynylestradiol (Cat# E4876), and alamethicin (Cat# E1127) were obtained from MilliporeSigma. ß-Estradiol 3-(β-D-glucuronide) sodium salt (Cat# HY-W585842) was purchased from MedChemExpress LLC.

    Nucleic Acid Electrophoresis:

    Article Title: MSC Exosomes and Rutin‐Chitosan–Pectin Nanoparticles Synergize to Ameliorate Adjuvant Arthritis via Th1/Th2 Modulation, MMP Suppression, Nrf2 Upregulation, and Antioxidant Boost
    Article Snippet: Protein extraction was performed using the ReadyPrep Protein Isolation Kit (Bio‐Rad Inc., Hercules, CA, USA), and protein concentrations were determined with the Bradford assay (Bio Basic Inc., Markham, Ontario, Canada). .. Equal amounts of protein (20 μg) were mixed with Laemmli sample buffer and denatured by boiling at 95°C for 5 min. Proteins were separated by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS‐PAGE) using the TGX Stain‐Free FastCast Acrylamide Kit (Bio‐Rad Laboratories, Inc., Hercules, CA, USA). .. The proteins were transferred to a PVDF membrane using the Bio‐Rad Trans‐Blot Turbo system.

    Clinical Proteomics:

    Article Title: Exploring the inflammatory origins of gouty arthritis: mechanistic studies based on local lesion proteomics and validation
    Article Snippet: Ammonium bicarbonate (Sigma-Aldrich, A6141-500G), TEAB (Sigma-Aldrich, T7408-100mL), Urea (Amresco, M123-1KG), Protein Quantification Dye (Huaxingbio, HXJ5137), Bovine Serum Albumin (Thermo Scientific, 23209), Dithiothreitol DTT (Amresco, M109-5G), Iodoacetamide IAM (Amresco, M216-30G), Trypsin (Promega, V5280/100ug), Ziptip (Millipore, ZTC18M096), Acetonitrile (J.T.Baker, 34851 MSDS), Ammonia solution (Wako Pure Chemical Industries Ltd, 013-23355), Formic acid (Sigma-Aldrich, T79708 ), Sample vials (Thermo, 11190533), Vial caps (Thermo, 11150635). .. Plasma Low Abundance Enrichment Magnetic Bead Kit QLBIO MagicOmics-DMB8X, Blood Exosome Enrichment Magnetic Bead Kit QLBIO MagicOmics-EMB8X, Micro/Universal Proteomics Kit QLBIO MagicOmics-MMB8X, Proteomics Robot QLBIO MagicOmics-AP-96, TGX Stain-Free FastCast Acrylamide Kit(Bio-Rad, #1610183), Universal Antibody Dilution Buffer (Yamei PS119L), anti-arachidonate 15-lipoxygenase(anti-ALOX15,BBI LIFE SCIENCES CORPORATION D161433), anti-glutathione peroxidase 4(anti-GPX4,Bioswamp PAB47937 ), ferritin heavy chain 1(anti-FTH1,BBI LIFE SCIENCES CORPORATION D290553), goat anti-rabbit IgG (BBI LIFE SCIENCES CORPORATION D110058), ECL Chemiluminescent Reagent (Millipore WBKLS0500). ..

    SDS Page:

    Article Title: Spliceosomal mutation drives melanoma tumorigenesis via lineage-specific RAS activation
    Article Snippet: Cells were seeded 48 hours before lysis and treated with 2% SDS (Sangon Biotech, no. B548118). .. Protein extracts were prepared by mixing with 6× SDS-PAGE Sample Loading Buffer (Beyotime, no. P0015F) and heating at 100°C for 10 min. Gels were cast using the TGX Stain-Free FastCast Acrylamide Kit, 10% (Bio-Rad, no. 1610183) ( ). .. Proteins were transferred using 1× transfer buffer prepared with a 1:1:3 dilution of water, absolute ethanol, and Trans-Blot Turbo 5× Transfer Buffer (Bio-Rad, no. 10026938) onto 0.22-μm Immun-Blot PVDF Membranes (Bio-Rad, no. 1620177).



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